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KMID : 0381120100320050463
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Genes and Genomics
2010 Volume.32 No. 5 p.463 ~ p.468
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Development of peptide nucleic acid (PNA) microarray for identification of Panax species based on the nuclear ribosomal internal transcribed spacer (ITS) and 5.8S rDNA regions
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Lee Jei-Wan
Bang Kyong-Hwan
Choi Jae-Jin
Chung Jong-Wook
Lee Jeong-Hoon
Jo Ick-Hyun
Seo A-Yeon
Kim Young-Chang
Kim Ok-Tae
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Abstract
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This study describes the identification of Panax species using a peptide nucleic acid (PNA) microarray. P. ginseng, P. quienquefolius, and P. japonicus were distinguished from each other using 5 PNA probes designed based on three single nucleotide polymorphisms (SNPs) detected in internal transcribed spacer (ITS) and 5.8S rDNA regions. Signal intensity comparison between PNA and DNA microarrays revealed that the PNA microarray provides a significantly more stable and specific fluorescent signal intensity than the DNA microarray. Three Panax species identified by the PNA microarray were denoted as barcode numbers depending on their fluorescent signal patterns of each species using 5 PNA probes (PG-ITS-116, PG-ITS-414-1, PG-ITS-414-2, PG-ITS-425-1, and PG-ITS-425-2). P. ginseng, P. quinquefolius, and P. japonicus were denoted as ‘11010’, ‘00202’ and ‘00000’, respectively. The PNA microarray developed in this study will be useful for legitimizing the distribution of ginseng in domestic and foreign ginseng markets.
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KEYWORD
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Panax ginseng, Internal transcribed spacer, 5.8S ribosomal DNA, Peptide nucleotide acid, Microarray, single nucleotide polymorphism
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